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Isolation protokol af musen monocyt-afledte dendritiske celler og deres efterfølgende In Vitro aktivering med Tumor immunkomplekser

Article DOI: 10.3791/57188-v • 11:48 min • May 31st, 2018
May 31st, 2018 •
*1, *1, 1, 1, 1, 2, 1
1Department of Pathology, Sackler School of Medicine, Tel-Aviv University, 2Department of Pathology, School of Medicine, Stanford University
* These authors contributed equally

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Monocyt-afledte DC (MoDC) kan føle mindre mængder af fare-associerede molekyler og er derfor let primet. Vi leverer en detaljeret protokol for dyrkning af MoDC fra blodet og tumorer og deres aktivering med immunkomplekser samtidig fremhæve vigtige forholdsregler, der bør overvejes for at undgå deres for tidlig aktivering.

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Kræftforskning spørgsmålet 135 immunkomplekser dendritiske celler Tumor-associerede DC monocyt-afledte DC kort kinaser tyrosin phosphatase Fcγ receptorer
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