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JoVE Journal Bioengineering
Una gotita-enfoque basado en microfluidos y amplificación por PCR de microesfera para amplicones de ADN
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Una gotita-enfoque basado en microfluidos y amplificación por PCR de microesfera para amplicones de ADN

Article DOI: 10.3791/57703-v • 11:40 min • November 14th, 2018
November 14th, 2018 •
*1, *2, 2, 3, 3, 1, 2,3, 1
1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University
* These authors contributed equally

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Este trabajo proporciona un método para la fabricación de plataformas de microfluídica basada en la gota y la aplicación de microesferas de poliacrilamida para la amplificación por PCR de la microesfera. El método de microesfera-PCR hace posible obtener amplicones de ADN monocatenario sin la separación del ADN de doble hebra.

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Bioingeniería número 141 copolimerización microfluidos ssDNA de canal enfoque flujo microesfera-PCR amplificación PCR asimétrico
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