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Quantitative Immunoblotting Zelllinien als Standard, Immunfluoreszenz zur Quantifizierung der Biomarker Proteine in Routine Gewebeproben zu validieren

Article DOI: 10.3791/58735-v • 09:58 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 3, 1,2
1Department of Pathology and Molecular Medicine, Queen's University, 2Division of Cancer Biology and Genetics, Queen's Cancer Research Institute, 3Queen's Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine, Queen's University

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Wir beschreiben die Verwendung von quantitativen Immunoblotting, Immunfluoreszenz Histologie gepaart mit Bildanalyse als Mittel zur Quantifizierung eines Proteins des Interesses in Formalin fixiert, Paraffin-eingebetteten (FFPE) Gewebeproben zu validieren. Unsere Ergebnisse zeigen den Nutzen der Immunfluoreszenz Histologie für die Ermittlung der relativen Menge Biomarker Proteine im Routine-Biopsie Proben.

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Biologie Ausgabe 143 Immunfluoreszenz Formalin fixierten Paraffin-eingebetteten quantitative Immunoblot western-Blot Pathologie Protein Biomarker Histologie Immunhistologie
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