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Escherichia coli-basert Cell-Free proteinsyntese: protokoller for en robust, fleksibel og tilgjengelig plattform-teknologi

Article DOI: 10.3791/58882-v • 09:45 min • February 25th, 2019
February 25th, 2019 •
*1,2, *2,3, 4,5,6,7, 2,3, 2,3
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University
* These authors contributed equally

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Denne protokollen detaljer trinn, kostnader, og nødvendig å generere E. coliutstyr-basert celle ekstrakter og implementere i vitro protein syntese reaksjoner innen 4 dager eller mindre. For å utnytte denne plattformen bred Søknadsfrist fleksibel natur, diskutere vi reaksjonen forhold som kan tilpasses og optimalisert.

Tags

Kjemi problemet 144 Cell-free protein syntese CFPS i vitro protein uttrykk høy gjennomstrømming protein syntese TX-TL syntetisk biologi Cell-free metabolske engineering CFME
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