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Hochdurchsatz-Nitrobenzoxadiazole-Label Cholesterin-Efflux-Assay

Article DOI: 10.3791/58891-v • 08:18 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1
1Group of Genomics and Pharmacogenomics in HIV, Laboratory of Retrovirology and Viral Immunopathogenesis, IDIBAPS, Hospital Clínic de Barcelona

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Messung der in-vitro-Cholesterin Efflux Fähigkeit, Serum oder Plasma in Makrophagen Zellmodellen ist ein viel versprechendes Instrument als Biomarker für Atherosklerose. In der vorliegenden Studie wir optimieren und standardisieren eine fluoreszierende NBD-Cholesterin-Efflux-Methode und eine Hochdurchsatz-Analyse mit 96-Well Platten zu entwickeln.

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Medizin Ausgabe 143 NBD-Cholesterin Makrophagen HDL Cholesterin-Efflux Fluoreszenz Apolipoprotein B-abgereicherte Serum Atherosklerose umgekehrte Cholesterin-Transport
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