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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering och kvantitativ utvärdering av pensel celler från mus Tracheas
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Isolering och kvantitativ utvärdering av pensel celler från mus Tracheas

Article DOI: 10.3791/59496-v • 10:25 min • June 12th, 2019
June 12th, 2019 •
1, 1, 1, 1
1Division of Rheumatology, Allergy and Immunology, Brigham and Women's Hospital, Harvard Medical School

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Pensel celler är sällsynta kolinerga kemosensoriska epitelceller som finns i den naiva mus luftstrupen. På grund av deras begränsade antal, ex vivo utvärdering av deras funktionella roll i luftvägarna immunitet och Remodeling är utmanande. Vi beskriver en metod för isolering av trakealborst celler med flödescytometri.

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Immunologi och infektion luftrör pensel celler kolinerga epitelceller ensamma kemosensoriska celler bitter smak Sensing celler bitter smak receptorer tofs celler
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