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Journal / Biochemistry / 富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定…
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Biochemistry

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富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定
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富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定

Article DOI: 10.3791/59729-v • 10:21 min • June 20th, 2019
June 20th, 2019 •
1,2, 1,2, 1,2, 1, 1, 3, 3, 4, 1,2
1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria

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用实例描述了含紫酯水酶的表达和纯化(在大肠杆菌、FPLC中的表达)。纯化蛋白用于结晶和抗体生产,并用于酶测定。提出了选定的光度测定法,以显示FAHD1的多功能性,如牛酸脱碳酶和丙烯酰胺水酶。

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生物化学 第148期 FAH FAHD FAHD1 ODx ApH 水酶 脱碳酰酶 牛乙酸酯 乙酰丙酸酯 紫霉素,丙烯酸盐 草酸盐 丙酸酯 米达斯
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