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Ein ektopisches Chemokin-Expressionsmodell zum Testen der Makrophagenrekrutierung in Vivo

Article DOI: 10.3791/60161-v • 10:02 min • September 25th, 2019
September 25th, 2019 •
1, 1, 2
1Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, 2Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology

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Um die Wirkung eines Chemokins auf die Makrophagenrekrutierung in vivo zu testen, wurde die gesamte Mount-in-situ-Hybridisierung verwendet, um die ektopische Expression des Chemokins zu erkennen, und Immunstaining wurde verwendet, um Makrophagen zu kennzeichnen. Live-Bildgebung wurde für die Echtzeitbeobachtung der Makrophagenmigration verwendet.

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Entwicklungsbiologie Ausgabe 151 fluoreszierende In-situ-Hybridisierung Immunostainierung Live-Bildgebung Zebrafisch IL-34 Makrophagen
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