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Journal / Cancer Research / 纤维细胞衍生的三维矩阵系统适用于内皮管形成测定…
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纤维细胞衍生的三维矩阵系统适用于内皮管形成测定
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纤维细胞衍生的三维矩阵系统适用于内皮管形成测定

Article DOI: 10.3791/60304-v • 07:21 min • December 26th, 2019
December 26th, 2019 •
1, 2, 3, 1, 4, 1
1Medical Oncology Department, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, 2Department of Medical Oncology, Hospital Universitario Puerta de Hierro de Majadahonda, 3Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC, 4Department of Oncology & Pathology, Karolinska Institutet

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此方法的目的是获得成纤维细胞衍生的 3D 基质作为后续细胞测定的天然支架。纤维细胞在预处理培养板中播种，并用抗坏血酸刺激，用于基质生成。基质被去细胞化并阻断以培养相关细胞（例如，内皮细胞）。

Tags

癌症研究， 问题 154， 肿瘤微环境， 三维 （3D） 培养， 细胞外基质， 纤维细胞， 胶原蛋白， 新血管生成
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