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Journal / Biochemistry / Kwantitatieve analyse van de cellulaire lipidoom…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Kwantitatieve analyse van de cellulaire lipidoom van Saccharomyces Cerevisiae met behulp van vloeibare chromatografie in combinatie met tandem massaspectrometrie
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Kwantitatieve analyse van de cellulaire lipidoom van Saccharomyces Cerevisiae met behulp van vloeibare chromatografie in combinatie met tandem massaspectrometrie

Article DOI: 10.3791/60616-v • 08:56 min • March 8th, 2020
March 8th, 2020 •
1, 2, 1, 1
1Department of Biology, Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry, Concordia University

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We presenteren een protocol met behulp van vloeibare chromatografie in combinatie met tandem massaspectrometrie te identificeren en te kwantificeren belangrijke cellulaire lipiden in Saccharomyces cerevisiae. De beschreven methode voor een kwantitatieve beoordeling van belangrijke lipideklassen binnen een gistcel is veelzijdig, robuust en gevoelig.

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Biochemie lipiden cellulaire lipidoom ongeesterte (vrije) vetzuren glycerophosfolipiden neutrale lipiden sfingolipiden ceramides cardiolipinen lipideextractie lipidomics vloeibare chromatografie massaspectrometrie
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