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Bioengineering

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Präklinische Kardiale Elektrophysiologie Bewertung durch Dual Voltage und Calcium Optical Mapping of Human Organotypic Cardiac Slices
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Präklinische Kardiale Elektrophysiologie Bewertung durch Dual Voltage und Calcium Optical Mapping of Human Organotypic Cardiac Slices

Article DOI: 10.3791/60781-v • 09:35 min • June 16th, 2020
June 16th, 2020 •
*1, *1, 1
1Department of Biomedical Engineering, The George Washington University
* These authors contributed equally

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Dieses Protokoll beschreibt das Verfahren zum Schneiden und Kultivieren menschlicher Herzscheiben für präklinische Arzneimitteltests und beschreibt die Verwendung von optischen Kartierungen zur gleichzeitigen Aufzeichnung von Transmembranspannung und intrazellulären Calciumsignalen aus diesen Scheiben.

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Bioengineering Ausgabe 160 menschliches Herz organotypische Kultur Herzscheiben optische Kartierung Elektrophysiologie Spannung Kalzium Arzneimitteltests
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