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Bioengineering

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Eine benutzerdefinierte Multiphotonen-Mikroskopie-Plattform für Live-Bildgebung von Maus-Cornea und Konjunktiva

Article DOI: 10.3791/60944-v • 06:53 min • May 17th, 2020
May 17th, 2020 •
1, 1, 2,3, *1,3,4,5, *6,7
1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University
* These authors contributed equally

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Präsentiert wird hier eine mikroskopische Multiphotonen-Plattform für die Live-Maus-Augenoberflächenbildgebung. Fluoreszierende transgene Maus ermöglicht die Visualisierung von Zellkernen, Zellmembranen, Nervenfasern und Kapillaren innerhalb der Augenoberfläche. Nichtlineare Signale der zweiten harmonischen Generation, die von kollagenen Strukturen abgeleitet werden, bieten eine etikettenfreie Bildgebung für stromale Architekturen.

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Bioengineering Ausgabe 159 Hornhaut Bindehaut Augenoberfläche Multiphotonenmikroskopie MPM intravitale Bildgebung transgene Maus
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