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Neuroscience

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JoVE Journal Neuroscience
Disección y aislamiento de la glía murina de múltiples regiones del sistema nervioso central
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Disección y aislamiento de la glía murina de múltiples regiones del sistema nervioso central

Article DOI: 10.3791/61345-v • 08:00 min • June 4th, 2020
June 4th, 2020 •
1, 1,2
1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute, Kent State University

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Aquí presentamos un protocolo para el aislamiento in vitro de múltiples poblaciones de células gliales de un SNC de ratón. Este método permite la segregación de microglía regional, células precursoras de oligodendrocitos y astrocitos para estudiar los fenotipos de cada uno en una variedad de sistemas de cultivo.

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Neurociencia Número 160 glía heterogeneidad regional célula precursora de oligodendrocitos microglía astrocito cultivo in vitro inmunocitoquímica
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