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Journal / Immunology and Infection / Fordøyelse av hele museøyne for…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Fordøyelse av hele museøyne for multiparameterflyt cytometrisk analyse av mononukleære phagocytes
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Fordøyelse av hele museøyne for multiparameterflyt cytometrisk analyse av mononukleære phagocytes

Article DOI: 10.3791/61348-v • 09:58 min • June 17th, 2020
June 17th, 2020 •
1, *2, *1
1Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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Denne protokollen gir en metode for å fordøye hele øynene inn i en enkelt celle suspensjon med det formål å multi-parameter flyt cytometrisk analyse for å identifisere spesifikke okulære mononukleære fagocytiske populasjoner, inkludert monocytter, microglia, makrofager, og dendrittiske celler.

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Immunologi og infeksjon utgave 160 øye strømningscytometri makrofag microglia monocytt myeloid
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