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Aislamiento de núcleos de tumores cerebrales frescos congelados para RNA-seq de un solo núcleo y ATAC-seq

Article DOI: 10.3791/61542-v • 06:22 min • August 25th, 2020
August 25th, 2020 •
1, 2, 1, 1,3, 4, 2, 1
1Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, 2Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), 3Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 4Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany

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La heterogeneidad intramoral es una característica inherente de los tumores, incluidos los gliomas. Desarrollamos un protocolo simple y eficiente que utiliza una combinación de tampones y centrifugación por gradiente para aislar núcleos individuales de tejidos de glioma frescos congelados para estudios de secuenciación de ARN de núcleo único y ATAC.

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Investigación del cáncer Número 162 Aislamiento de núcleos snRNA-seq snATAC-seq Genómica 10x Glioma
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