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Journal / Biochemistry / Krystallisering og strukturel bestemmelse af et…
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Biochemistry
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JoVE Journal Biochemistry
Krystallisering og strukturel bestemmelse af et enzym
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
Journal (Biochemistry)
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
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Journal (Biochemistry)
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Krystallisering og strukturel bestemmelse af et enzym:Substratkompleks ved seriel krystallografi i en alsidig mikrofluidisk chip

Article DOI: 10.3791/61972-v • 10:45 min • March 20th, 2021
March 20th, 2021 •
1,4, 1,2, 3, 2, 1, 1, 1, 1, 2, 2, 1
1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

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En alsidig mikrofluidisk enhed beskrives, der muliggør krystallisering af et enzym ved hjælp af modspredningsmetoden, indførelsen af et substrat i krystallerne ved iblødsætning og 3D-strukturbestemmelsen af enzymet:substratkomplekset ved en seriel analyse af krystaller inde i chippen ved stuetemperatur.

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Biokemi Problem 169 krystallisering seriel krystallografi 3D-struktur modspredning ChipX mikrofluidics CCA-tilsættende enzym iblødsætning såning
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