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Erzeugung von murinen primären Dickdarmepithel-Monoschichten aus Darmkrypten
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Erzeugung von murinen primären Dickdarmepithel-Monoschichten aus Darmkrypten

Article DOI: 10.3791/62156-v • 06:57 min • February 6th, 2021
February 6th, 2021 •
1, 1, 1, 1, 1
1Department of Pathology, School of Medicine, University of Michigan

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In diesem Protokoll beschreiben wir, wie man murine primäre epitheliale Dickdarmmonoschichten direkt aus Darmkrypten erzeugt. Wir bieten experimentelle Ansätze zur Erzeugung konfluenter Monoschichten auf permeablen Filtern, konfluente Monoschichten für die Kratzwundeheilung und biochemische Studien sowie spärliche und konfluente Monoschichten für die Immunfluoreszenzanalyse.

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Biologie Ausgabe 168 Primärkultur 2D-Enteroide 2D-Koloreide Epithelzellen epitheliale Monoschichten Darmkrypten
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