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JoVE Journal Cancer Research

Ett permanent fönster för att undersöka cancermetastasering till lungan

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

Journal (Cancer Research)

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Journal (Cancer Research)

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

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Ett permanent fönster för att undersöka cancermetastasering till lungan

Article DOI: 10.3791/62761-v • 07:06 min • July 1st, 2021

July 1st, 2021 •

Lucia Borriello*^1,2, Brian Traub*^1,2,3, Anouchka Coste^1,2,3, Maja H. Oktay^1,2,4,5, David Entenberg^1,2,4

¹Department of Anatomy and Structural Biology, Einstein College of Medicine / Montefiore Medical Center, ²Gruss-Lipper Biophotonics Center, Einstein College of Medicine / Montefiore Medical Center, ³Department of Surgery, Einstein College of Medicine / Montefiore Medical Center, ⁴Integrated Imaging Program, Einstein College of Medicine / Montefiore Medical Cente, ⁵Department of Pathology, Einstein College of Medicine / Montefiore Medical Center

* These authors contributed equally

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Här presenterar vi ett protokoll för kirurgisk implantation av ett permanent indwelling optiskt fönster för murine bröstkorgen, som möjliggör högupplöst, intravital avbildning av lungan. Fönstrets persistens gör det väl lämpat för studier av dynamiska cellulära processer i lungan, särskilt de som långsamt utvecklas, såsom metastaserad progression av spridda tumörceller.

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Cancerforskning nummer 173

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