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Immunology and Infection

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JoVE Journal Immunology and Infection
Un sistema de cultivo automatizado para su uso en pruebas preclínicas de terapias dirigidas al huésped para la tuberculosis
Paraffin Embedding and Histopathological Scoring of Demyelinating Lesions and Perivascular Cuffs in Mouse Brain and Spinal Cord Tissues (Video) | JoVE
Journal (Immunology and Infection)
Paraffin Embedding and Histopathological Scoring of Demyelinating Lesions and Perivascular Cuffs in Mouse Brain and Spinal Cord Tissues (Video) | JoVE
Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis
Journal (Immunology and Infection)
Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis
 
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Un sistema de cultivo automatizado para su uso en pruebas preclínicas de terapias dirigidas al huésped para la tuberculosis

Article DOI: 10.3791/62838-v • 09:34 min • August 16th, 2021
August 16th, 2021 •
1, 1,2, 2, 2,3,4, 1, 1
1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, 2School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland

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La cuantificación rápida y eficiente del crecimiento intracelular de M. tuberculosis es crucial para buscar mejores terapias contra la tuberculosis (TB). Este protocolo describe un ensayo de detección colorimétrica basado en caldo utilizando un sistema automatizado de cultivo líquido para cuantificar el crecimiento de Mtb en macrófagos tratados con terapias candidatas dirigidas al huésped.

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Inmunología e infección Número 174
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