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Caracterização de Junções Neuromusculares emundongos por Microscopia Confocal Combinada e Super-Resolução…
JoVE Journal
Neuroscience
Neuroscience
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Caracterização de Junções Neuromusculares em Camundongos por Microscopia Confocal Combinada e Super-Resolução
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Article
DOI:
10.3791/63032-v
•
11:03 min
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December 8th, 2021
Caracterização de Junções Neuromusculares em Camundongos por Microscopia Confocal Combinada e Super-Resolução
December 8th, 2021
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Martina Marinello*1,2, Jérémie Cosette*1, Caroline Bogni1,2, Jérôme Denard1,2, Daniel Stockholm*1,3, Ana Buj-Bello*1,2
1Genethon, 91000, Evry, France, 2Université Paris-Saclay, Univ Evry, Inserm, Genethon, Integrare research unit UMR_S951, 91000, Evry, France, 3Ecole Pratique des Hautes Etudes, PSL Research University, 75014, Paris, France
* These authors contributed equally
챕터
요약
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Este protocolo descreve um método para análise morfométrica de junções neuromusculares por microscopia confocal combinada e STED que é usado para quantificar alterações patológicas em modelos de camundongos de SMA e CMS relacionada ao ColQ.