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Bioengineering

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JoVE Journal Bioengineering
Großflächige, automatisierte Produktion von aus Fett gewonnenen Stammzellsphäroiden für den 3D-Bioprinting
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Großflächige, automatisierte Produktion von aus Fett gewonnenen Stammzellsphäroiden für den 3D-Bioprinting

Article DOI: 10.3791/63430-v • 07:40 min • March 31st, 2022
March 31st, 2022 •
1,2,3, 1,2,4, 1,2,4, 1,2, 2,3,4,5, 1,2,3,4
1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense

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Hier beschreiben wir die großtechnische Produktion von aus Fett gewonnenen Stroma-/Stammzell-Sphäroiden (ASC) unter Verwendung eines automatisierten Pipettiersystems zur Aussaat der Zellsuspension, wodurch die Homogenität der Sphäroidgröße und -form sichergestellt wird. Diese ASC-Sphäroide können als Bausteine für 3D-Bioprinting-Ansätze verwendet werden.

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Bioengineering Ausgabe 181
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