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Reconstitution de cortex minimembranaires d’actine sur des bicouches lipidiques soutenues

Article DOI: 10.3791/63968-v • 11:55 min • July 12th, 2022
July 12th, 2022 •
1, 2, 2, 2
1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, 2National Centre for Biological Sciences, Tata Institute of Fundamental Research

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Ce protocole décrit la formation de bicouches lipidiques soutenues et l’ajout de filaments cytosquelettiques et de protéines motrices pour étudier la dynamique de réseaux cytosquelettiques reconstitués et attachés à la membrane à l’aide de la microscopie à fluorescence.

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Biologie numéro 185
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