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JoVE Journal Biology
Isolierung, Charakterisierung und therapeutische Anwendung extrazellulärer Vesikel aus kultivierten humanen mesenchymalen Stammzellen
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Isolierung, Charakterisierung und therapeutische Anwendung extrazellulärer Vesikel aus kultivierten humanen mesenchymalen Stammzellen

Article DOI: 10.3791/64135-v • 07:03 min • September 23rd, 2022
September 23rd, 2022 •
1,2, 2,3, 2,4, 2,5, 2,5, 2, 2, 2,3
1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3Xi'an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine, Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology, Fourth Military Medical University

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Das vorliegende Protokoll beschreibt die differentielle Zentrifugation zur Isolierung und Charakterisierung repräsentativer EVs (Exosomen und Mikrovesikel) aus kultivierten humanen MSCs. Weitere Anwendungen dieser Elektrofahrzeuge werden ebenfalls in diesem Artikel erläutert.

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Biologie Heft 187
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