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JoVE Journal Biology

Kültürlenmiş İnsan Mezenkimal Kök Hücrelerinden Hücre Dışı Veziküllerin İzolasyonu, Karakterizasyonu ve Terapötik Uygulaması

Isolation and Culture of Avian Embryonic Valvular Progenitor Cells

Journal (Biology)

Isolation and Culture of Avian Embryonic Valvular Progenitor Cells

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Journal (Biology)

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

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Kültürlenmiş İnsan Mezenkimal Kök Hücrelerinden Hücre Dışı Veziküllerin İzolasyonu, Karakterizasyonu ve Terapötik Uygulaması

Article DOI: 10.3791/64135-v • 07:03 min • September 23rd, 2022

September 23rd, 2022 •

Shu-Juan Xing^1,2, Kai-Chao Zhang^2,3, Si-Yuan Tang^2,4, Lu Liu^2,5, Yuan Cao^2,5, Chen-Xi Zheng², Bing-Dong Sui², Yan Jin^2,3

¹College of Life Science, Northwest University, ²State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, ³Xi'an Institute of Tissue Engineering and Regenerative Medicine, ⁴School of Basic Medicine, Fourth Military Medical University, ⁵Department of Orthodontics, School of Stomatology, Fourth Military Medical University

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Mevcut protokol, temsili EV'leri (eksozomlar ve mikro-parçacıklar) kültürlü insan MSC'lerinden izole etmek ve karakterize etmek için diferansiyel santrifüjlemeyi açıklamaktadır. Bu EV'lerin diğer uygulamaları da bu makalede açıklanmaktadır.

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