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Genetics

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Adipocytspecifik ATAC-Seq med fedtvæv ved hjælp af fluorescensaktiveret kernesortering

Article DOI: 10.3791/65033-v • 11:11 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine

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Vi præsenterer en protokol til assay for transposase-tilgængeligt kromatin med high-throughput sekventering (ATAC-seq) specifikt på adipocytter ved hjælp af kernesortering med fedtvæv isoleret fra transgene reportermus med nuklear fluorescensmærkning.

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Denne måned i JoVE nummer 193
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