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JoVE Journal Biology
通过 CLON-G 和 体外 自发死亡测定延长中性粒细胞寿命
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通过 CLON-G 和 体外 自发死亡测定延长中性粒细胞寿命

Article DOI: 10.3791/65132-v • 05:52 min • May 12th, 2023
May 12th, 2023 •
*1,2, *3, *4, 1,2, 5, 1,2, 5
1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 2Tianjin Institutes of Health Science, 3Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 4Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, 5Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center
* These authors contributed equally

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该协议详细介绍了CLON-G的制备，以将中性粒细胞寿命延长至5天以上，并为使用流式细胞术和共聚焦荧光显微镜评估中性粒细胞死亡提供了可靠的程序。

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生物学，第195期，
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