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Voorbereiding en Fractionering van Xenopus laevis Egg Extracten
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Voorbereiding en Fractionering van Xenopus laevis Egg Extracten

Article DOI: 10.3791/891-v • 07:45 min • August 27th, 2008
August 27th, 2008 •
1, 1
1Department of Cell Biology, Emory University

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Ruwe en gefractioneerde Xenopus ei-extracten kan worden gebruikt om ingrediënten voor reconstructie van cellulaire processen voor morfologische en biochemische analyses. Ei lysis en differentiële centrifugatie worden gebruikt om het ruwe extract die op hun beurt gebruikt worden om in gefractioneerde extracten en licht membraan voorbereidingen voor te bereiden voor te bereiden.

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Cellular Biology Current Protocols Wiley Xenopus laevis Egg extracten dichtheidsgradiënt centrifugatie Licht membraanfractie Nucleaire Fraction
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