HIGH SCHOOL

Biology

Science Experiments

Basic Biology

Cell Counting with a Hemacytometer Grid

Monitoring Cell Line Health and Re-Seeding

PCR Steps and Reaction Setup

Running a DNA Gel Electrophoresis Test

Analyzing Protein Size by SDS-PAGE

Heat Shock for Plasmid DNA Uptake

Making Electrocompetent Bacterial Cells

Protein Detection with ELISA

Plasmid DNA Cleanup from Bacterial Cells

Purifying DNA From Agarose Gels

Protein Detection by Western Blot

How DNA and RNA Enter Mammalian Cells

DNA Ligase in Cloning and Repair

Restriction Enzymes: DNA Cutting Guide

Molecular Cloning in Bacteria

This is a free preview. For full access

Basic Methods in Cellular and Molecular Biology

Plasmid DNA Cleanup from Bacterial Cells

Description

Plasmid DNA cleanup from bacterial cells is a lab method for separating plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA molecule that carries a specific DNA fragment. After transformation into a host organism, the plasmid is replicated and ma...

Show More

Transcript

Plasmiden zijn circulaire, extrachromosomale DNA-moleculen die in de moleculaire biologie dienen als dragers, of vectoren, voor een specifiek DNA-fragment. Bacteriën worden gebruikt om plasmiden te repliceren, zodat het DNA van belang op grote schaal kan worden geproduceerd. Het proces waarmee onderzoekers plasmiden uit bacte...

Show More

Tags

Bacteri le kweek DNA isolatie anionenuitwisseling silicacolom spectrophotometrische analyse gelelektroforese miniprep procedure transfectietoepassing affiniteitszuivering

More videos by Standards

Show More