Lipid Vesicle Rupture
Lipid Vesicle Rupture - Related Videos
08:35
Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
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Cited by 16 •
2014
Microfluidic jetting against a droplet interface lipid bilayer provides a reliable way to generate vesicles with control over membrane asymmetry, incorporation of transmembrane proteins, and encapsulation of material. This technique can be applied to study a variety of biological systems where compartmentalized biomolecules are desired.
05:16
A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture
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2023
AlPcS2a-mediated chromophore-assisted laser inactivation (CALI) is a powerful tool for studying spatiotemporal damage of intracellular vesicles (IVs) in live cells.
07:33
Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
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Cited by 14 •
2011
To test the interaction of a protein with its target lipid we used MACS and Annexin V-conjugated magnetic beads and lipid vesicles synthesized from the target lipid and Annexin V-binding phosphatidylserine. Proteins bound to the target lipid are co-purified and analyzed after elution from the beads.
11:35
Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
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Cited by 29 •
2012
This protocol describes an extrusion method for preparing lipid vesicles of sub-micron sizes with a high degree of homogeneity. This method uses a pressure-controlled system with controlled nitrogen flow rates for liposome preparation. The lipid preparation1,2, liposome extrusion, and size characterization will be presented herein.
03:36
Dissipative Microgravimetry Technique to Study Protein-Lipid Bilayer Interaction
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2025
This video demonstrates a dissipative microgravimetry technique to investigate the binding of proteins to lipid bilayers. Small unilamellar vesicles are added to coat the surface of the quartz sensor of a microbalance. Utilizing the calcium ion-dependent binding of the target phospholipid-binding protein to the bilayer, changes in the oscillation frequency of the sensor and the dissipation of the oscillation are monitored to determine the nature of the protein-lipid bilayer binding.
FAQs
Rupture begins when vesicles adsorb to a suitable substrate and contact with that surface deforms the bilayer. Continued interaction generates membrane tension, which can destabilize the vesicle, promote pore formation, and allow the lipid layer to spread. Adsorption is therefore not merely a positioning step; it initiates the mechanical sequence that produces a planar membrane arrangement.
Pore formation provides a transition between deformation and spreading. As tension develops in the adsorbed bilayer, localized openings can form. These pores allow lipid material to reorganize across the substrate rather than remain confined to an individual vesicle. Their formation helps explain how a three-dimensional vesicle becomes part of a continuous planar layer.
Substrate choice matters because vesicle adsorption and subsequent bilayer spreading depend on interactions with the surface. A suitable surface supports the tension-driven sequence described for Lipid Vesicle Rupture. If the interaction does not support deformation, pore formation, and spreading, the intended planar membrane layer may not form, limiting the technique's usefulness.
An intact vesicle presents lipids in a closed, three-dimensional structure, whereas a supported lipid bilayer provides a planar membrane layer at a surface. This change creates a controlled format for examining membrane structure and function. The planar arrangement is particularly useful when experiments require surface-based observation or interactions involving proteins, transported molecules, or biosensor components.
The workflow begins by bringing lipid vesicles into contact with a suitable surface so they can adsorb. Surface interactions then deform the vesicles and generate membrane tension. Pores form as the bilayer destabilizes, followed by lipid spreading across the substrate. The resulting supported layer can serve as the membrane model for later structural or functional experiments.
These model membranes support studies of protein-lipid interactions, membrane permeability, and molecular transport. They also provide a platform for surface-based biosensors. Because the membrane is formed under controlled laboratory conditions, researchers can investigate these processes in a simplified system that retains important membrane features without requiring the full complexity of a biological cell membrane.