Intestinal Barrier Model
Intestinal Barrier Model - Related Videos
08:58
Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
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Cited by 18 •
2017
Current treatments of inflammatory bowel diseases (IBD) aim at alleviating disease symptoms but may cause severe side effects. Thus, alternative strategies are being investigated in animal colitis models. Here, we explain how the beneficial effects of diet supplements on clinical IBD signs are analyzed in such a colitis model.
07:18
Investigating Intestinal Barrier Breakdown in Living Organoids
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Cited by 13 •
2020
Here we describe a technique to quantify the barrier integrity of small intestinal organoids. The fact that the method is based on living organoids enables the sequential investigation of different barrier integrity modulating substances or combinations thereof in a time-resolved manner.
04:58
Mouse Ileal Loop Model: A Surgical Model to Study Intestinal Permeability in the Mouse
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2025
This video demonstrates the generation of an ileal loop model to study intestinal permeability in a mouse model. This method provides a live model to understand the epithelial barrier function and consequent immune response.
09:23
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
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Cited by 31 •
2017
The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
FAQs
Cell–cell junctions regulate how readily substances pass between neighboring intestinal epithelial cells, while transport processes through the cells contribute to selective movement across the model. Researchers can examine changes in permeability to determine whether barrier regulation has been maintained or disrupted. This helps connect cellular organization with intestinal health, disease-related changes, and transport behavior.
Mucus provides an additional barrier component, while selective transport determines which substances can move across the epithelial layer. Considering both features gives a more complete representation of intestinal function than examining cell passage alone. Their inclusion can help researchers study nutrient or drug movement and evaluate how barrier properties influence interactions with microbes or immune components.
Microbe or immune components introduce biological interactions that are not captured by epithelial cells alone. These additions allow researchers to investigate how intestinal barrier behavior is coordinated with surrounding biological signals and how inflammation or infection may affect permeability. The resulting system can provide more relevant context for studying disease-associated barrier disruption while retaining controlled experimental conditions.
More advanced models are intended to improve physiological relevance by representing a broader range of intestinal features or interactions than a basic epithelial-cell system. This can strengthen the interpretation of permeability, inflammation, infection, and transport studies. Such models are particularly useful when researchers need experimental findings to better reflect complex intestinal conditions and support therapeutic development.
Researchers grow intestinal epithelial cells under controlled conditions, establish the relevant barrier features, and then assess movement across the model. Permeability measurements can reveal whether substances cross selectively or whether the barrier has become disrupted. Investigators may compare these outcomes under conditions related to inflammation, infection, drug exposure, or nutrient transport, depending on the study goal.
This model is useful when the research question concerns how a drug or nutrient interacts with intestinal barrier properties or moves across an epithelial layer. Controlled testing can provide information about transport and possible effects on permeability. The approach supports early investigation of absorption-related behavior and can help evaluate candidates before further therapeutic or biological studies.
Studies can measure permeability, characterize selective transport, and investigate changes associated with inflammation or infection. Models may also help examine interactions among epithelial cells, mucus, microbes, and immune components. These outcomes support research into intestinal health and disease, while more physiologically relevant systems can contribute to therapeutic development by providing a controlled platform for comparing responses.