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Investigating Intestinal Barrier Breakdown in Living Organoids
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Medicine
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JoVE Journal Medicine
Investigating Intestinal Barrier Breakdown in Living Organoids

Investigating Intestinal Barrier Breakdown in Living Organoids

DOI:
10.3791/60546-v

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07:18 min

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March 26, 2020

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, , , , , , ,
1Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center,Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 2Department of Medicine 1,Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 3Optical Imaging Centre Erlangen (OICE),Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg

Chapters

  • 00:05Introduction
  • 00:57Barrier Integrity Measurement Preparation
  • 03:04Organoid Permeability Assay
  • 05:39Results: Representative Intestinal Organoid Barrier Integrity Analysis
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a technique to quantify the barrier integrity of small intestinal organoids. The fact that the method is based on living organoids enables the sequential investigation of different barrier integrity modulating substances or combinations thereof in a time-resolved manner.

Tags

Keywords: Intestinal BarrierOrganoidsBarrier IntegrityTight Junction ProteinsEpithelial BarrierCell CulturePermeability AssayInterferon Gamma

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