HIGH SCHOOL

Biology

Science Experiments

Basic Biology

Cell Counting with a Hemacytometer Grid

Monitoring Cell Line Health and Re-Seeding

PCR Steps and Reaction Setup

Running a DNA Gel Electrophoresis Test

Analyzing Protein Size by SDS-PAGE

Heat Shock for Plasmid DNA Uptake

Making Electrocompetent Bacterial Cells

Protein Detection with ELISA

Plasmid DNA Cleanup from Bacterial Cells

Purifying DNA From Agarose Gels

Protein Detection by Western Blot

How DNA and RNA Enter Mammalian Cells

DNA Ligase in Cloning and Repair

Restriction Enzymes: DNA Cutting Guide

Molecular Cloning in Bacteria

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Basic Methods in Cellular and Molecular Biology

Heat Shock for Plasmid DNA Uptake

Description

Bacterial transformation lets cells take up foreign DNA from their surroundings. Some bacteria can do this naturally. In molecular biology, researchers recreate the process in the lab by making pores in the bacterial cell membrane.

Cells that can take up DNA from the environment are called compet...

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Transcript

A transformação bacteriana é um método amplamente utilizado onde o DNA estranho é introduzido em uma bactéria, que pode então amplificar, ou clonar o DNA. Células que têm a capacidade de facilmente pegar este DNA são chamadas células competentes. Embora a transformação esteja ocorrendo naturalmente em muitos tipos de bacté...

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Tags

C lulas Competentes DNA Plasmidial Cloreto de C lcio Resist ncia a Antibi ticos Contagem de Col nias Efici ncia de Transforma o T cnica Ass ptica Triagem Azul Branco

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