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Basic Methods in Cellular and Molecular Biology
Making Electrocompetent Bacterial Cells
Description
Bacterial transformation can be done by making electrocompetent cells. In this process, foreign DNA enters bacterial cells after an electrical field creates small pores in the cell wall. The cells that can take up DNA from the environment are called competent cells. In the laboratory, scientists can produce electrocompetent ...
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Transcript
A transformação bacteriana é um processo natural, no qual as bactérias ingerem DNA estranho e depois amplificam ou clonam. Em laboratório, esse processo pode ser induzido artificialmente, usando pulsos de campo elétricos de alta tensão para criar poros na membrana celular bacteriana, através da qual o DNA plasmídeo pode pas...
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