HIGH SCHOOL

Biology

Science Experiments

Basic Biology

Cell Counting with a Hemacytometer Grid

Monitoring Cell Line Health and Re-Seeding

PCR Steps and Reaction Setup

Running a DNA Gel Electrophoresis Test

Analyzing Protein Size by SDS-PAGE

Heat Shock for Plasmid DNA Uptake

Making Electrocompetent Bacterial Cells

Protein Detection with ELISA

Plasmid DNA Cleanup from Bacterial Cells

Purifying DNA From Agarose Gels

Protein Detection by Western Blot

How DNA and RNA Enter Mammalian Cells

DNA Ligase in Cloning and Repair

Restriction Enzymes: DNA Cutting Guide

Molecular Cloning in Bacteria

This is a free preview. For full access

Basic Methods in Cellular and Molecular Biology

Purifying DNA From Agarose Gels

Description

Purifying DNA from agarose gels lets students recover a separated DNA fragment after electrophoresis. The DNA is first isolated from the gel, then moved through a silica column for cleanup.

The process has three main steps: binding, washing, and eluting. DNA is believed to bind to silica when hig...

Show More

Transcript

A purificação de gel é um procedimento padrão realizado para recuperar fragmentos de DNA desejados de géis agarose após separação eletroforética. Depois de dissolver o fragmento de gel e executá-lo através de um filtro especializado, este procedimento produz DNA livre de impurezas como sais, nucleotídeos livres e enzimas, ad...

Show More

Tags

Recupera o de DNA Coluna de S lica Liga o Lavagem Elui o Gel de Agarose Coluna de Centrifuga o Lavagem com Etanol Elui o de DNA Solubiliza o de Gel Aplica es Posteriores

More videos by Standards

Show More