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同心凝胶系统来研究基质微环境的三维细胞迁移的生物物理作用
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JoVE Journal Bioengenharia
Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
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同心凝胶系统来研究基质微环境的三维细胞迁移的生物物理作用

DOI:
10.3791/52735-v

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11:43 min

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April 03, 2015

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, ,
1Systems Biophysics Department,FOM Institute AMOLF, 2Mechanobiology Institute,National University of Singapore, 3Department of Biomedical Engineering,National University of Singapore

Capítulos

  • 00:05Título
  • 01:22Harvesting Cells
  • 02:32Preparing the Collagen Solutions
  • 03:52Formation of Concentric Gel Cultures
  • 06:54Live-Cell Imaging of Concentric Gel Cultures
  • 08:16Tracking Cell Movement
  • 09:38Results: Cell Movement Analysis
  • 11:03Conclusion

Summary

Tadução automática

Tadução automática

机械性能和细胞外基质的组织强烈地影响细胞的三维迁移。 的体外方法来研究在生物物理变量的环境中，在人口和单个细胞水平的时空细胞迁移行为，进行说明。

Tags

3D Cell MigrationCell InvasionExtracellular MatrixMechanosensingBreast Cancer CellsCollagen GelsLive-cell ImagingBiophysical Microenvironment

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