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An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

Article DOI: 10.3791/53575-v • 08:40 min • March 14th, 2016
March 14th, 2016 •
1, 1,2,3, 4, 5, 4,6, 4, 7, 4, 4, 1,8
1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, and Swiss Institute of Bioinformatics, 3Swiss Institute of Bioinformatics, 4Li Ka Shing Institute for Virology, University of Alberta, 5Regional Infectious Diseases Unit, University of Edinburgh, 6Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 7Infection Biology, Department of Biomedicine, University of Basel, 8Clinical Microbiology, University Hospital Basel

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The presented protocols describe two enzyme-linked immunosorbent assay (ELISA) based techniques for the rapid investigation of ligand-receptor interactions: The first assay allows the determination of dissociation constant between ligand and receptor. The second assay enables a rapid screening of blocking peptides for ligand-receptor interactions.

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ELISA-based Method Binding Affinity Ligand-receptor Interactions Inhibitory Peptides Protein-protein Interactions Cytokine-receptor Binding Technique Advantages Label Receptors ELISA Reader Carbonate Coating Buffer Ligand Or Peptide Concentrations Direct Ligand Receptor Interaction ELISA Receptor-ligand Dissociation Constant Competition Ligand-receptor Interaction ELISA
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