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1Singapore Immunology Network (SIgN),Agency for Science, Technology and Research (A*STAR), Biopolis, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 3Lee Kong Chian School of Medicine,Nanyang Technological University, 4Centenary Institute for Cancer Medicine and Cell Biology, 5Discipline of Dermatology,University of Sydney, 6Department of Dermatology,Royal Prince Alfred Hospital, 7LSI Immunology Programme,National University of Singapore, 8School of Biological Sciences,Nanyang Technological University
Capítulos
- 00:05Título
- 00:53Mouse Anesthesia and Depilation
- 01:35Induction of Ischemia and Reperfusion Injury
- 02:31Placement of the Ear on the Imaging Platform
- 04:33Results: Neutrophil Recruitment in Ischemia-reperfusion Injury
- 05:20Conclusion
Denne protokol beskriver induktionen af en iskæmi-reperfusion (IR) model på museøre huden ved hjælp af magnet fastspænding. Ved hjælp af en specialbygget intravital imaging model, vi studerer in vivo inflammatoriske reaktioner post-reperfusion. Rationalet bag udviklingen af denne teknik er at udvide forståelsen af, hvordan leukocytter reagerer på huden IR skade.
