Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Charlotte S.L. Bailey; Robert A. Bone; Philip J. Murray; J. Kim Dale

doi:10.3791/55127

Journal

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Biologia do Desenvolvimento

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Ordenação temporal de Expressão Dynamic Data de expressão espacial mapas detalhados

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

JoVE Journal
Biologia do Desenvolvimento

This content is Free Access.

JoVE Journal Biologia do Desenvolvimento

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Ordenação temporal de Expressão Dynamic Data de expressão espacial mapas detalhados

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5,874 Views

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11:52 min

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February 09, 2017

DOI:

10.3791/55127-v

DOI:

10.3791/55127-v

•

11:52 min

February 09, 2017

•

3 Views

Charlotte S.L. Bailey*¹, Robert A. Bone*¹, Philip J. Murray², J. Kim Dale³

¹The Danish Stem Cell Center (DanStem),University of Copenhagen, ²Division of Mathematics,University of Dundee, ³Division of Cell and Developmental Biology, College of Life Sciences,University of Dundee

Transcrição

Summary

The segmentation clock drives oscillatory gene expression across the pre-somitic mesoderm (PSM). Dynamic Notch activity is key to this process. We use imaging and computational analyses to extract temporal dynamics from spatial expression data to demonstrate that Delta ligand and Notch receptor expression oscillate in the vertebrate PSM.