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Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
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Medicina
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JoVE Journal Medicina
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

DOI:
10.3791/55192-v

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08:43 min

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February 14, 2017

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, , , , , ,
1Department of Intensive Care Medicine,University Hospital Aachen, 2Institute of Biochemistry and Molecular Biology,University Hospital Aachen, 3Department of Radiology,German Cancer Research Center, 4Department of Thoracic and Cardiovascular Surgery,University Hospital Aachen, 5Department of Vascular Biology, Institute for Stroke and Dementia Research (ISD),Klinikum der Universität München, 6Deutsches Zentrum für Herz-/Kreislaufkrankheiten (DZHK),Munich Heart Alliance

Capítulos

  • 00:05Título
  • 01:11Isolation of Endothelial Progenitor Cells (EPCs)
  • 03:52Migration Assay
  • 06:50Results: Characterization of Endothelial Progenitor Cells (EPCs)
  • 08:20Conclusion

Summary

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Tadução automática

Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.

Tags

Endothelial Progenitor CellsCardiac SurgeryCell IsolationMigrationCD-34 Positive CellsDensity GradientPeripheral Blood Mononuclear CellsMagnetic BeadsCell CultureAngiogenesisIschemiaRe-perfusion Injury

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