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Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Article DOI: 10.3791/55280-v • 10:21 min • June 29th, 2017
June 29th, 2017 •
1, 2, 2, 3, 1, 1, 2
1Department of Chemical Engineering, McGill University, 2Michael Smith Laboratories & Department of Chemical and Biological Engineering, University of British Columbia, 3Michael Smith Laboratories & Department of Pharmaceutical Sciences, University of British Columbia

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This video and manuscript describe an emulsion-based method to encapsulate mammalian cells in 0.5% to 10% alginate beads which can be produced in large batches using a simple stirred vessel. The encapsulated cells can be cultured in vitro or transplanted for cellular therapy applications.

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Mammalian Cell Encapsulation Alginate Beads Stirred Vessel Immobilize Cells Water And Oil Emulsion Internal Gelation Aqueous Phase Mineral Oil Organic Phase Acidify Emulsion Oil-soluble Acid Acetic Acid PH Drop Calcium Carbonate Dissolution Recovery Of Beads In Vitro Culture Transplantation Nozzle-based Cell Encapsulators Enzyme Immobilization Microbial Cell Immobilization Alginate Encapsulation Immunoisolation
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