Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases

Maneesh Dave; Paola Menghini; Keiki Sugi; Rodrigo A. Somoza; Zhenghong Lee; Mukesh Jain; Arnold Caplan; Fabio Cominelli

doi:10.3791/55367

Guiada por ultra-som intracardíaca injeção de células-tronco mesenquimais humanas para aumentar H...

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Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases

Guiada por ultra-som intracardíaca injeção de células-tronco mesenquimais humanas para aumentar Homing para o intestino para uso em modelos murino de doenças intestinais inflamatórias Experimental

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07:45 min

September 1, 2017

DOI: 10.3791/55367-v

Maneesh Dave¹, Paola Menghini¹, Keiki Sugi^2,3, Rodrigo A. Somoza⁵, Zhenghong Lee⁴, Mukesh Jain^2,3, Arnold Caplan⁵, Fabio Cominelli¹

¹Division of Gastroenterology and Liver Disease, University Hospitals, Digestive Health Research Institute,Case Western Reserve University, ²Case Cardiovascular Research Institute, School of Medicine,Case Western Reserve University, ³Department of Medicine, Harrington Discovery Institute, Harrington Heart and Vascular Institute,University Hospitals Case Medical Center, ⁴Department of Radiology,University Hospitals Case Medical Center, ⁵Department of Biology - Skeletal Research Center,Case Western Reserve University

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Overview

This study investigates the use of ultrasound-guided intracardiac injections of human bone marrow-derived mesenchymal stem cells (HMSCs) in a mouse model of Crohn's disease. The goal is to enhance the localization of HMSCs to the inflamed intestine, addressing key questions in inflammatory bowel disease research.

Key Study Components

Area of Science

Neuroscience
Stem Cell Research
Inflammatory Bowel Disease

Background

Mesenchymal stem cells (MSCs) have shown potential in treating colonic inflammation.
Previous studies indicate low homing efficiency of MSCs to inflamed tissues.
Ultrasound guidance may improve the delivery of HMSCs to target areas.
Understanding MSC localization is crucial for developing effective therapies.

Purpose of Study

To evaluate the effectiveness of ultrasound-guided intracardiac injections of HMSCs.
To determine the homing efficiency of HMSCs to the intestine during inflammation.
To assess the impact of this method on mouse mobility and mortality.

Methods Used

Ultrasound-guided intracardiac injection of HMSCs in mice.
Monitoring of HMSC localization in the intestine post-injection.
Assessment of mouse health and mobility during the procedure.
Analysis of the success rate of HMSC delivery to the target area.

Main Results

Ultrasound guidance significantly improved HMSC localization to the inflamed intestine.
The method demonstrated a high success rate with minimal adverse effects on mice.
Findings contribute valuable insights into MSC therapy for inflammatory bowel disease.
Challenges in the procedure highlight the need for thorough training in HMSC biology.

Conclusions

Ultrasound-guided intracardiac injection is a promising technique for MSC delivery.
Improved localization of HMSCs may enhance therapeutic outcomes in Crohn's disease.
Further research is needed to optimize this method for clinical applications.

Frequently Asked Questions

What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into various cell types and have potential therapeutic applications.

How does ultrasound guidance improve HMSC delivery?

Ultrasound guidance allows for precise targeting of the injection site, enhancing the localization of HMSCs to the inflamed areas.

What is the significance of HMSC homing to the intestine?

HMSC homing to the intestine is crucial for developing effective treatments for inflammatory bowel diseases, as it may improve healing and reduce inflammation.

What challenges are associated with this injection method?

Challenges include the need for thorough understanding of HMSC biology and the variability in procedure outcomes due to multiple influencing factors.

Who conducted this study?

The study was conducted by Paola Menghini, a postdoctoral researcher in the laboratory.

What are the potential applications of this research?

This research may lead to improved MSC therapies for inflammatory bowel diseases and other conditions requiring targeted cell delivery.

Murino estudos em modelos de inflamação do cólon têm demonstrado que uma pequena porcentagem (1-5%) de células-tronco mesenquimais (MSC) injetada por via intravenosa ou intraperitonealmente home ao cólon inflamado¹^,². Este estudo mostra que guiada por ultra-som intracardíacos injeções de MSCs resultam em aumentada localização até o intestino.

O objetivo geral deste procedimento é usar células-tronco mesenquimais derivadas da medula óssea humana intracardíaca guiadas por ultrassom ou injeção de HMSC em um modelo de camundongo da doença de Crohn experimental, para observar o homing do HMSC ao intestino. Este método pode ajudar a responder às principais questões no campo da doença inflamatória intestinal, sobre a necessidade de aumentar a localização do HMSC no intestino delgado durante a inflamação. A principal vantagem dessa técnica é que ela facilita uma alta taxa de sucesso de entrega de HMSC com mobilidade e mortalidade mínimas do camundongo.

Geralmente, indivíduos novos neste método podem ter problemas porque várias variáveis podem afetar o resultado do procedimento. Exigindo muita compreensão do estagiário da biologia das células-tronco mesenquimais humanas. Demonstrando os procedimentos estará Paola Menghini, pós-doutoranda do nosso laboratório.

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