JoVE Journal
Biochemistry
Biochemistry
This Conteúdo is Open Access.
Capítulos
Resumo
Structure determination of macromolecular complexes using cryoEM has become routine for certain classes of proteins and complexes. Here, this pipeline is summarized (sample preparation, screening, data acquisition and processing) and readers are directed towards further detailed resources and variables that may be altered in the case of more challenging specimens.