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JoVE Journal Biochemistry
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
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Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Article DOI: 10.3791/63333-v • 12:07 min • February 18th, 2022
February 18th, 2022 •
1, 1, 1, 1, 1
1Research Institute for Biomedical Aging Research, University of Innsbruck

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This protocol describes how to extract fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) from swine kidney and mouse liver. The listed methods may be adapted to other proteins of interest and modified for other tissues.

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Extraction Purification FAHD1 Protein Swine Kidney Mouse Liver Recombinant Protein Bacteria Protein Lysis Buffer Sodium Fluoride Phenylmethylsulfonyl Fluoride Aprotinin Activated Orthovanadate Tissue Sample Ammonium Sulfate Solution
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