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Ekstraktion og oprensning af FAHD1-protein fra svinenyre og muselever
Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
Journal (Biochemistry)
Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
Journal (Biochemistry)
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Ekstraktion og oprensning af FAHD1-protein fra svinenyre og muselever

Article DOI: 10.3791/63333-v • 12:07 min • February 18th, 2022
February 18th, 2022 •
1, 1, 1, 1, 1
1Research Institute for Biomedical Aging Research, University of Innsbruck

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Denne protokol beskriver, hvordan man ekstraherer fumarylacetoacetathydrolasedomæneholdigt protein 1 (FAHD1) fra svinenyre og muselever. De anførte metoder kan tilpasses andre proteiner af interesse og modificeres til andre væv.

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Biokemi udgave 180 FAHD FAHD1 oxaloacetat decarboxylase ODx væv FPLC kromatografi proteinekstraktion
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