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Low-Input-Nukleus-Isolierung und Multiplexing mit Barcode-Antikörpern sympathischer Mausganglien für die Single-Nucleus-RNA-Sequenzierung
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Low-Input-Nukleus-Isolierung und Multiplexing mit Barcode-Antikörpern sympathischer Mausganglien für die Single-Nucleus-RNA-Sequenzierung

Article DOI: 10.3791/63397-v • 10:44 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1, 1,2, 1, 2, 1, 3, 1,2
1Department of Anatomy & Embryology, Leiden University Medical Center, 2Department of Cardiology, Leiden University Medical Center, 3Department of Medical Statistics and Bioinformatics, Leiden University Medical Center

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Dieses Protokoll beschreibt die detaillierte, inputarme Probenvorbereitung für die Einzelkernsequenzierung, einschließlich der Dissektion von Maus-Superior-Cervix- und Sternganglien, Zelldissoziation, Kryokonservierung, Nukleusisolierung und Hashtag-Barcoding.

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Medizin Ausgabe 181
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