Myeloid Cell Migration
Myeloid Cell Migration - Related Videos
05:14
Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
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2025
Given the limited xenograft models available to study interactions between human CART and myeloid cells, we established in vitro and in vivo models to understand the impacts of human macrophages on CART cells. Findings can potentially be generalized to evaluate macrophage roles in the tumor microenvironment and test macrophage-targeted immunotherapies.
06:39
Culture of myeloid dendritic cells from bone marrow precursors
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Cited by 22 •
2008
This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.
09:07
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Cited by 27 •
2013
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
10:21
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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Cited by 3 •
2018
Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.
08:52
An Introduction to Cell Motility and Migration
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2023
Cell motility and migration play important roles in both normal biology and in disease. On one hand, migration allows cells to generate complex tissues and organs during development, but on the other hand, the same mechanisms are used by tumor cells to move and spread in a process known as cancer metastasis. One of the primary cellular machineries that make cell movement possible is an intracellular network of myosin and actin molecules, together known as “actomyosin”, which creates a...
FAQs
Chemokine gradients create changing concentration cues across blood vessels and tissues. Myeloid cells detect these differences and move toward regions associated with infection, injury, or inflammation rather than dispersing randomly. The direction and strength of this guidance help determine which cells reach an affected site and how effectively they can participate in the local immune response.
Integrin activation enables myeloid cells to adhere to the endothelium, the cell layer lining blood vessels. This adhesion is essential because circulating cells must attach securely before crossing the vessel wall. Integrins therefore connect chemical guidance from inflammatory mediators with the physical step of entering affected tissue, allowing migration to proceed beyond the bloodstream.
Neutrophils, monocytes, and macrophages are all included among the myeloid cells recruited by physiological or pathological signals, but their trafficking can be examined as distinct cellular responses. Studying these populations separately helps clarify which cells reach infected or injured tissue and how their recruitment may contribute to inflammation, autoimmune disease, or tumor progression.
Migration assays and imaging approaches provide complementary ways to examine immune-cell trafficking. Assays help evaluate how cells respond to recruitment-related conditions, whereas imaging can show movement in relation to blood vessels or affected tissue. Together, these approaches allow investigators to study directed recruitment and assess therapeutic strategies intended to modify immune-cell localization or tissue damage.
Assessing recruitment can reveal how innate immune cells reach sites of infection, injury, and inflammation. It can also indicate whether trafficking is associated with beneficial immune responses or with pathological processes such as chronic inflammation, autoimmune disease, and tumor progression. These observations support evaluation of how changes in cell movement may influence tissue damage and disease behavior.
Medical research examines this process because controlling immune-cell recruitment could influence inflammation and tissue injury. Migration studies help investigators evaluate therapeutic strategies that modulate which myeloid cells enter affected tissues and how extensively they accumulate there. This relevance extends across infection, injury, autoimmune disease, chronic inflammation, and tumor progression, where cell trafficking can shape outcomes.