Fume Hoods and Laminar Flow Cabinets

Lab Safety

Your institution must subscribe to JoVE's Basic Biology collection to access this content.

Fill out the form below to receive a free trial or learn more about access:

Welcome!

Enter your email below to get your free 1 hour trial to JoVE!





By clicking "Submit", you agree to our policies.

 

Overview

Robert M. Rioux & William A. Elliott, Pennsylvania State University, University Park, PA

Fume hoods and laminar flow cabinets are engineering controls that operate under similar principles. Both use a constant flow of air to prevent contamination of the laboratory environment and its inhabitants. Fume hoods prevent hazardous substances from exiting the hood workspace, whereas laminar flow cabinets prevent contaminants from entering the cabinet workspace.

Fume hoods are ventilation systems designed to minimize exposure to hazardous vapors, fumes, and particles. A constant flow of air is drawn into the hood opening, limiting the escape of vapors, fumes, and particles, and then is pulled out through the exhaust. Laminar flow cabinets are used to maintain a sterile/clean environment by constantly flowing high-efficiency particulate arrestance (HEPA)-filtered air outwards, minimizing contaminated air entering the cabinet workspace. The HEPA-filtered air reduces the opportunity for harmful chemicals or particles from entering the laboratory. A HEPA filter removes 99.97% or greater of 0.3 µm particles.

Cite this Video

JoVE Science Education Database. Lab Safety. Fume Hoods and Laminar Flow Cabinets. JoVE, Cambridge, MA, (2017).

Principles

Fume hoods and laminar flow cabinets are engineering controls that aim to reduce exposure to hazards and contamination. Fume hoods reduce exposure to hazardous vapors, fumes, and particles for the user while laminar flow cabinets reduce workspace exposure to contaminants. Turbulent flow follows an irregular flow pattern with local flow moving in all directions relative to the bulk flow. Laminar flow moves in parallel streamlines that do not cross. Laminar flow cabinets maintain a laminar stream of air to prevent cross contamination within the workspace and to prevent backflow of contaminated air from outside the hood that would occur with turbulent flow.

Procedure

1. Fume hoods

  1. Uses
    1. Fume hoods are used when a material generates harmful vapors, fumes, or airborne particles, such as fine silica powders, or volatile carcinogens, such as benzene.
  2. Operation
    1. Air is drawn in though the opening face of the hood, where the user works, and out through the exhaust. The constant flow of air inwards towards the face prevents hazardous vapors, fumes, and particles from escaping out through the hood opening, keeping the user and other laboratory workers safe.
  3. Facial flow velocity must be high enough for the hood to be effective. A low flow velocity allows harmful fumes, vapors, or particles to escape through the opening of the hood toward the user. One cause of low flow velocity is having the adjustable window at the hood opening, called a sash, too high. It is common for fume hoods to have low flow velocity alarms and sash height alarms. Typical flow velocities are between 0.41 and 0.51 m/s (ANSI/AIHA/ASSE Z9.5). Hoods should have the maximum safe working height of the sash clearly marked.
  4. There are several rules for safe use of fume hoods.
    1. Never put your head into the hood space, since inserting your head inside the hood can expose you to harmful materials. The hood is designed to protect users from chemical exposure only when utilized properly. Only the users' arms should be present in the hood. Wear appropriate personal protective equipment (PPE) at all times, regardless of the protection afforded by the fume hood. Consult with your organization's Environmental Health & Safety (EHS) office for proper PPE recommendations, if they are unknown.
    2. Always work with the sash at or below the maximum safe height.
    3. When not in use, the sash should be closed. Closing the sash ensures a safer working environment for all laboratory occupants. Additionally, the energy costs associated with improper fume hood operation are immense. Maintaining the sash height at the minimum, non-working level is more energy efficient.
    4. Do not use the hood for chemical storage. Store chemicals in appropriate locations, such as a flammable cabinet, and bring them into the fume hood only when needed.
    5. Place all materials in the fume hood at least 6 inches away from the edge of the fume hood face. When work is carried out within 6 inches of the edge, vapors, fumes, and particles are more likely to escape.
    6. Just as good housekeeping principles apply on working laboratory benches, the same principles should be practiced within fume hoods.
  5. Perform regular maintenance on a fume hood to ensure it is operating safely. Maintenance should include testing of alarms and testing flow velocity at the design operating sash position. Many factors can affect the flow velocity, including flow patterns within the room the hood is located and obstructions at the exhaust vent. If flow velocity is low at the design operating sash position, lower the sash until the flow velocity is at the required speed. Many modern fume hoods have air flow velocity monitors that monitor the flow velocity in real time. If the sash becomes too low for effective work to be performed at the fume hood, cease operation until the root of the problem is addressed.
  6. Fume Hood Variations
    1. There are number of types of more specialized fume hoods that may be encountered. These include perchloric acid hoods, radioisotope hoods, ductless hoods, and others. More can be read about the requirements for these hoods in ANSI/AIHA/ASSE Z9.5.

2. Laminar flow cabinets

  1. Uses
    1. Laminar flow cabinets are used when a clean environment, free of particles or biological contaminants, is required. Common examples include working with tissue cultures or semiconductor wafers. Laminar flow cabinets prevent airborne contamination from entering the cabinet workspace. The laminar flow (as opposed to turbulent flow) of air minimizes cross-contamination of samples within the cabinet.
  2. Air is filtered by a HEPA filter and blown over the workspace, out toward the user. A constant outward flow of clean air maintains an uncontaminated workspace. Some laminar flow cabinets are fitted with a UV-C lamp to disinfect the workspace prior to use.
  3. There are two types of laminar flow cabinets: horizontal flow and vertical flow ( Figure 1). Horizontal flow cabinets blow clean air from the back face of the cabinet horizontally toward the user, whereas vertical flow hoods blow clean air from the ceiling of the cabinet toward the floor of the workspace where it then hits the base and flows in a horizontal direction towards the user. Vertical flow cabinets are used with the sash pulled down. Horizontal flow cabinets do not have a sash. Both types of laminar flow cabinets have their advantages and disadvantages.

Figure 1
Figure 1. Diagrams of horizontal and vertical laminar flow hoods.

Horizontal Laminar Flow Hood
Advantages Disadvantages
Hands/gloves contaminate less as they are typically downwind of items in the cabinet Air stream blows in user’s face
Reduced air flow turbulence Large objects on workspace can obstruct flow of clean air, reducing effectiveness
Vertical Laminar Flow Hood
Advantages Disadvantages
Air stream does not blow in user’s face Cannot position hands and arms above objects
Less cross contamination from item in workspace Increased air flow turbulence

Table 1. Advantages and disadvantages of horizontal and vertical laminar flow hoods.

  1. Tips to ensure effective use of laminar flow cabinets.
    1. Always take care not to put items downstream of items that could lead to cross contamination. This is particularly relevant when using biological samples.
    2. Minimize clutter. The more items that are in the cabinet, the more likely contamination is to occur. Large items can disrupt flow.
    3. Ensure that hands/gloves and any items that are brought into the cabinet are free from contamination prior to entering the cabinet.
    4. All items must be placed 6 inches or further from the edge of the cabinet opening. The air within 6 inches of the edge is more likely to mix with the outside air, meaning objects within 6 inches are more likely to be contaminated.
    5. Regular maintenance should be performed on a laminar flow cabinet to ensure it is operating safely. Maintenance should include checking and replacing the HEPA filter, checking for leaks in the cabinet, and testing airflow velocity. The integrity of the HEPA filter should checked by testing the number of particles that pass through the filter. The filter should remove 99.97% or greater of 0.3 µm particles. If the flow velocity is too low, the cabinet will be ineffective in keeping out contaminants. If the flow velocity is too great, the flow will be turbulent, with contamination becoming more likely.

Fume hoods and laminar flow cabinets are essential pieces of laboratory equipment that prevent hazardous situations and contamination.

In fume hoods and laminar flow cabinets, hazards or contaminants are reduced using airflow. Fume hoods draw in air across a workspace to remove hazardous fumes and fine particles, while laminar flow cabinets blow air through a filter and out to prevent contamination of samples by dust or biological materials.

This video will illustrate how fume hoods and laminar flow cabinets operate, how to use them, and how to perform maintenance.

Fume hoods and laminar flow cabinets operate using laminar airflow, a flow that proceeds in parallel streamlines, which do not cross. Laminar flow, as opposed to turbulent flow, prevents cross-contamination between samples by flowing around objects removing hazardous particles.

Fume hoods have three major parts: the opening face with a sash, the workspace, and the exhaust. The fan in the exhaust draws in air through the opening face, across the workspace, and out through the exhaust. This flow in turn draws out fumes and particles out of the exhaust and away from the laboratory.

At its proper height, the sash restricts the size of the opening, which in turn maintains a high flow of air. This high flow is necessary to prevent the escape of fumes.

Meanwhile, there are two types of laminar flow cabinets, horizontal and vertical. In both, air is drawn through an intake and purifying filter where it is cleared of small particles like dust and bacteria.

A horizontal cabinet directs air horizontally through the workspace. This type of cabinet lessens contamination by hands and gloves, as they are downstream of the samples. However, the airstream does blow directly onto the user, and large objects can obstruct the flow.

In a vertical cabinet, the air is directed from above onto the workspace, then out past a sash. As this type of flow directly contacts the surface of the workspace materials, it helps to prevent cross-contamination. However, the sash can restrict hand movement and the airflow is more turbulent than in a horizontal cabinet.

Now, we will show you how to use these workspaces in a laboratory setting and how to perform basic maintenance.

To use a fume hood safely, always wear appropriate personal protective equipment. Raise the sash up only to the indicated maximum safe working height ensuring sufficient airflow through the hood.

To prevent exposure to harmful fumes or particles, work with only your arms inside the hood, and never let your head enter the workspace. Also, to make sure that the velocity is sufficient throughout the hood, keep the workspace uncluttered, and move all items at least six inches away from the hood face.

When you are done working in the hood, take out all materials. Do not store chemicals in the hood, but rather in a dedicated storage location like a flammables cabinet. Lastly, close the sash to ensure a safer laboratory environment and reduce energy usage.

Perform regular maintenance by testing alarms and flow velocity at the maximum sash height.

If the velocity is low, lower the sash until it is at the required speed. If the sash becomes too low for work to be done at the hood, cease operations until the root of the problem is addressed.

Laminar flow cabinets are commonly used in places where contamination is a concern, like a biology laboratory, so you will need to be careful of contamination by yourself and laboratory air. To prevent contamination, use ethanol to sterilize gloves and any equipment before using the cabinet or opening the sash.

Make sure the sash is not above the maximum allowed height to ensure a sufficient flow of air. Keep the cabinet free from clutter, and make sure objects are placed at least 6 inches from the edge, as that is the area most likely to be contaminated by laboratory air. Also, do not place items in danger of cross-contamination downstream from each other.

When you are done, remove all items from the workspace to prevent clutter and close the sash to prevent contamination. Then, if the cabinet is equipped with a UV-C light, turn it on to disinfect the workspace.

Perform regular maintenance on the laminar flow cabinet. Check for leaks by coating sensitive areas like the sash edges with a soap solution, which will bubble at the site of escaping air.

You've just watched JoVE's introduction to fume hoods and laminar flow cabinets. You should now understand how they work, how to use them, and how to perform maintenance. Thanks for watching!

Applications and Summary

Fume hoods and laminar flow cabinets are useful tools in the laboratory to prevent harm from hazardous materials and to keep a clean working space when using sensitive materials. However, fume hoods and laminar flow cabinets are only effective when used properly. Following simple operating guidelines and performing regular maintenance, fume hoods and laminar flow cabinets can be effective tools in the laboratory.

References

  1. American National Standard for Laboratory Ventilation," American National Standards Institute, Inc./ American Industrial Hygiene Association, ANSI/AIHA Z9.5, 2012.

1. Fume hoods

  1. Uses
    1. Fume hoods are used when a material generates harmful vapors, fumes, or airborne particles, such as fine silica powders, or volatile carcinogens, such as benzene.
  2. Operation
    1. Air is drawn in though the opening face of the hood, where the user works, and out through the exhaust. The constant flow of air inwards towards the face prevents hazardous vapors, fumes, and particles from escaping out through the hood opening, keeping the user and other laboratory workers safe.
  3. Facial flow velocity must be high enough for the hood to be effective. A low flow velocity allows harmful fumes, vapors, or particles to escape through the opening of the hood toward the user. One cause of low flow velocity is having the adjustable window at the hood opening, called a sash, too high. It is common for fume hoods to have low flow velocity alarms and sash height alarms. Typical flow velocities are between 0.41 and 0.51 m/s (ANSI/AIHA/ASSE Z9.5). Hoods should have the maximum safe working height of the sash clearly marked.
  4. There are several rules for safe use of fume hoods.
    1. Never put your head into the hood space, since inserting your head inside the hood can expose you to harmful materials. The hood is designed to protect users from chemical exposure only when utilized properly. Only the users' arms should be present in the hood. Wear appropriate personal protective equipment (PPE) at all times, regardless of the protection afforded by the fume hood. Consult with your organization's Environmental Health & Safety (EHS) office for proper PPE recommendations, if they are unknown.
    2. Always work with the sash at or below the maximum safe height.
    3. When not in use, the sash should be closed. Closing the sash ensures a safer working environment for all laboratory occupants. Additionally, the energy costs associated with improper fume hood operation are immense. Maintaining the sash height at the minimum, non-working level is more energy efficient.
    4. Do not use the hood for chemical storage. Store chemicals in appropriate locations, such as a flammable cabinet, and bring them into the fume hood only when needed.
    5. Place all materials in the fume hood at least 6 inches away from the edge of the fume hood face. When work is carried out within 6 inches of the edge, vapors, fumes, and particles are more likely to escape.
    6. Just as good housekeeping principles apply on working laboratory benches, the same principles should be practiced within fume hoods.
  5. Perform regular maintenance on a fume hood to ensure it is operating safely. Maintenance should include testing of alarms and testing flow velocity at the design operating sash position. Many factors can affect the flow velocity, including flow patterns within the room the hood is located and obstructions at the exhaust vent. If flow velocity is low at the design operating sash position, lower the sash until the flow velocity is at the required speed. Many modern fume hoods have air flow velocity monitors that monitor the flow velocity in real time. If the sash becomes too low for effective work to be performed at the fume hood, cease operation until the root of the problem is addressed.
  6. Fume Hood Variations
    1. There are number of types of more specialized fume hoods that may be encountered. These include perchloric acid hoods, radioisotope hoods, ductless hoods, and others. More can be read about the requirements for these hoods in ANSI/AIHA/ASSE Z9.5.

2. Laminar flow cabinets

  1. Uses
    1. Laminar flow cabinets are used when a clean environment, free of particles or biological contaminants, is required. Common examples include working with tissue cultures or semiconductor wafers. Laminar flow cabinets prevent airborne contamination from entering the cabinet workspace. The laminar flow (as opposed to turbulent flow) of air minimizes cross-contamination of samples within the cabinet.
  2. Air is filtered by a HEPA filter and blown over the workspace, out toward the user. A constant outward flow of clean air maintains an uncontaminated workspace. Some laminar flow cabinets are fitted with a UV-C lamp to disinfect the workspace prior to use.
  3. There are two types of laminar flow cabinets: horizontal flow and vertical flow ( Figure 1). Horizontal flow cabinets blow clean air from the back face of the cabinet horizontally toward the user, whereas vertical flow hoods blow clean air from the ceiling of the cabinet toward the floor of the workspace where it then hits the base and flows in a horizontal direction towards the user. Vertical flow cabinets are used with the sash pulled down. Horizontal flow cabinets do not have a sash. Both types of laminar flow cabinets have their advantages and disadvantages.

Figure 1
Figure 1. Diagrams of horizontal and vertical laminar flow hoods.

Horizontal Laminar Flow Hood
Advantages Disadvantages
Hands/gloves contaminate less as they are typically downwind of items in the cabinet Air stream blows in user’s face
Reduced air flow turbulence Large objects on workspace can obstruct flow of clean air, reducing effectiveness
Vertical Laminar Flow Hood
Advantages Disadvantages
Air stream does not blow in user’s face Cannot position hands and arms above objects
Less cross contamination from item in workspace Increased air flow turbulence

Table 1. Advantages and disadvantages of horizontal and vertical laminar flow hoods.

  1. Tips to ensure effective use of laminar flow cabinets.
    1. Always take care not to put items downstream of items that could lead to cross contamination. This is particularly relevant when using biological samples.
    2. Minimize clutter. The more items that are in the cabinet, the more likely contamination is to occur. Large items can disrupt flow.
    3. Ensure that hands/gloves and any items that are brought into the cabinet are free from contamination prior to entering the cabinet.
    4. All items must be placed 6 inches or further from the edge of the cabinet opening. The air within 6 inches of the edge is more likely to mix with the outside air, meaning objects within 6 inches are more likely to be contaminated.
    5. Regular maintenance should be performed on a laminar flow cabinet to ensure it is operating safely. Maintenance should include checking and replacing the HEPA filter, checking for leaks in the cabinet, and testing airflow velocity. The integrity of the HEPA filter should checked by testing the number of particles that pass through the filter. The filter should remove 99.97% or greater of 0.3 µm particles. If the flow velocity is too low, the cabinet will be ineffective in keeping out contaminants. If the flow velocity is too great, the flow will be turbulent, with contamination becoming more likely.

Fume hoods and laminar flow cabinets are essential pieces of laboratory equipment that prevent hazardous situations and contamination.

In fume hoods and laminar flow cabinets, hazards or contaminants are reduced using airflow. Fume hoods draw in air across a workspace to remove hazardous fumes and fine particles, while laminar flow cabinets blow air through a filter and out to prevent contamination of samples by dust or biological materials.

This video will illustrate how fume hoods and laminar flow cabinets operate, how to use them, and how to perform maintenance.

Fume hoods and laminar flow cabinets operate using laminar airflow, a flow that proceeds in parallel streamlines, which do not cross. Laminar flow, as opposed to turbulent flow, prevents cross-contamination between samples by flowing around objects removing hazardous particles.

Fume hoods have three major parts: the opening face with a sash, the workspace, and the exhaust. The fan in the exhaust draws in air through the opening face, across the workspace, and out through the exhaust. This flow in turn draws out fumes and particles out of the exhaust and away from the laboratory.

At its proper height, the sash restricts the size of the opening, which in turn maintains a high flow of air. This high flow is necessary to prevent the escape of fumes.

Meanwhile, there are two types of laminar flow cabinets, horizontal and vertical. In both, air is drawn through an intake and purifying filter where it is cleared of small particles like dust and bacteria.

A horizontal cabinet directs air horizontally through the workspace. This type of cabinet lessens contamination by hands and gloves, as they are downstream of the samples. However, the airstream does blow directly onto the user, and large objects can obstruct the flow.

In a vertical cabinet, the air is directed from above onto the workspace, then out past a sash. As this type of flow directly contacts the surface of the workspace materials, it helps to prevent cross-contamination. However, the sash can restrict hand movement and the airflow is more turbulent than in a horizontal cabinet.

Now, we will show you how to use these workspaces in a laboratory setting and how to perform basic maintenance.

To use a fume hood safely, always wear appropriate personal protective equipment. Raise the sash up only to the indicated maximum safe working height ensuring sufficient airflow through the hood.

To prevent exposure to harmful fumes or particles, work with only your arms inside the hood, and never let your head enter the workspace. Also, to make sure that the velocity is sufficient throughout the hood, keep the workspace uncluttered, and move all items at least six inches away from the hood face.

When you are done working in the hood, take out all materials. Do not store chemicals in the hood, but rather in a dedicated storage location like a flammables cabinet. Lastly, close the sash to ensure a safer laboratory environment and reduce energy usage.

Perform regular maintenance by testing alarms and flow velocity at the maximum sash height.

If the velocity is low, lower the sash until it is at the required speed. If the sash becomes too low for work to be done at the hood, cease operations until the root of the problem is addressed.

Laminar flow cabinets are commonly used in places where contamination is a concern, like a biology laboratory, so you will need to be careful of contamination by yourself and laboratory air. To prevent contamination, use ethanol to sterilize gloves and any equipment before using the cabinet or opening the sash.

Make sure the sash is not above the maximum allowed height to ensure a sufficient flow of air. Keep the cabinet free from clutter, and make sure objects are placed at least 6 inches from the edge, as that is the area most likely to be contaminated by laboratory air. Also, do not place items in danger of cross-contamination downstream from each other.

When you are done, remove all items from the workspace to prevent clutter and close the sash to prevent contamination. Then, if the cabinet is equipped with a UV-C light, turn it on to disinfect the workspace.

Perform regular maintenance on the laminar flow cabinet. Check for leaks by coating sensitive areas like the sash edges with a soap solution, which will bubble at the site of escaping air.

You've just watched JoVE's introduction to fume hoods and laminar flow cabinets. You should now understand how they work, how to use them, and how to perform maintenance. Thanks for watching!

A subscription to JoVE is required to view this article.
You will only be able to see the first 20 seconds.

RECOMMEND JoVE