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9.5:

Photosystem I

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Biology
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JoVE Core Biology
Photosystem I

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Similar to photosystem II, photosystem I also has the core domain called the P700 reaction center and a peripheral domain called the light harvesting complex. The electrons are transferred from PSII to PSI through a series of electron acceptor molecules, starting from an electron carrier– plastoquinone, followed by a cytochrome complex, and a mobile electron carrier– plastocyanin. Once the plastocyanin is reduced, it transfers an electron to the oxidized PSI reaction center. The reduced P700 then absorbs light and relays an excited electron to PSI's unique electron acceptor– ferredoxin. Finally, the electrons are accepted by ferredoxin-NADP+ reductase, ultimately forming the high energy product, NADPH. The transfer of electrons through the electron transport chain on the thylakoid membrane releases energy that is used to pump protons into the thylakoid space through the cytochrome complex, creating a proton gradient. ATP synthase, a specialized proton channel, utilizes this proton gradient to attach a third phosphate group to an ADP and produce ATP via chemiosmosis.

9.5:

Photosystem I

Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.

Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized by PSI. As a result, another photon is absorbed by the pigments of the PSI antenna complex and relayed to the PSI reaction center called P700. P700 is oxidized and sends a high-energy electron to NADP+ to form NADPH. Just as PSII captures the energy to create proton gradients to make ATP, PSI captures the energy to reduce NADP+ into NADPH.

After the energy from the sun is converted into chemical energy in the form of ATP and NADPH molecules, the cell has the fuel needed to build carbohydrate molecules for long-term energy storage in the stroma of the chloroplast.

Suggested Reading

  1. Cardona, T., Shao, S., Nixon, P.J. Enhancing Photosynthesis in Plants: The Light Reactions. Essays In Biochemistry. 62 (1), 85-94 (2018).