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26.16: Formation of Intermediate Filaments

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Cell Biology

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Formation of Intermediate Filaments

26.16: Formation of Intermediate Filaments

Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been reported.

The multi-step process uses two types of associations: lateral association, i.e., side-to-side binding, and longitudinal association, i.e., head-to-tail binding. The monomeric core is similar across different intermediate filaments with a tripartite structure, with an α-helical rod domain flanked by two non-α-helical domains. The formation starts with the lateral association of two monomers forming a dimer. Depending on the type of monomer, the dimer can be homodimeric, made up of identical monomers, or heterodimeric, made up of two different types of monomers. These dimers then associate in an antiparallel manner to form a tetramer. Eight tetramers associate laterally to form a bundle, called the unit length filament or ULF. The ULF is 60 nm in length and 10 nm in diameter. These unit-length filaments arrange in a head-to-tail manner to form the intermediate filaments. 

Suggested Reading


Intermediate Filaments Cytoskeletal Proteins Tensile Strength Flexibility Microfilaments Microtubules Enzymatic Activity Nucleotides ATP GTP Polymerization Self-assembly Accessory Proteins Lateral Association Longitudinal Association Monomeric Core Tripartite Structure Alpha-helical Rod Domain Non-alpha-helical Domains Dimer Homodimeric Heterodimeric Antiparallel Manner Tetramer Unit Length Filament (ULF) Bundle Head-to-tail Arrangement

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