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33.17: Electron Microscope Tomography and Single-particle Reconstruction
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Electron Microscope Tomography and Single-particle Reconstruction
 
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33.17: Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.

Electron Tomography

Electron tomography can be performed either in TEM or STEM (scanning transmission electron microscopy) mode. STEM is a technique primarily used for obtaining tomograms of thick biological specimens. It combines the sample surface scanning methodology of scanning electron microscopy, such that the electrons transmitted or scattered at each point where the electron beam hits the sample are collected by a series of detectors.

A variation of the conventional electron tomography technique is dual-axis tomography, where the sample is tilted around two axes with respect to the electron beam. This results in two separate tomograms that are then aligned to generate a 3D image of the sample. Dual-axis tomography has two advantages over single-axis tomography: a better reconstruction of the sample's expanded features and increased sample depth resolution.

Another variation is the electron cryo-tomography, in which samples are imaged under cryogenic conditions because fixation and dehydration can damage the biological structures. The method is primarily used for thin samples, less than 500 nm in thickness, because thick samples block the electron beam. Therefore, the technique has been limited to purified macromolecular complexes, viruses, or small cells such as bacterial cells.

Single-particle Reconstruction

Single-particle analysis is typically used with cryo-electron microscopy to generate 3D structures with near-atomic resolution. It is most suited for large or dynamic macromolecular complexes that are difficult to crystallize, making it a substitute for X-ray crystallography. For complexes that can be crystallized, single-particle analysis is a method of choice to obtain high-resolution structures. The technique has been used for studying membrane proteins, protein complexes, chromatin structure, and macromolecular machines such as ribosomes, and proteasomes.

Tags

Electron Microscope Tomography Single-particle Reconstruction TEM STEM Electron Tomography Scanning Transmission Electron Microscopy Sample Surface Scanning Dual-axis Tomography Electron Cryo-tomography 3D Structure Determination Macromolecules Macromolecular Complexes In Vitro Conditions In Vivo Conditions

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